A Simple and Sensitive RT-qPCR Technology for Rapid Detection of Porcine Reproductive and Respiratory Syndrome Virus.

ABSTRACT: To establish a rapid and sensitive detection method for the porcine reproductive and respiratory syndrome virus (PRRSV), gene-specific primers and a TaqMan probe were designed based on the M gene of PRRSV, and a new stable fully pre-mixed reverse transcription real-time fluorescence quantitative PCR (RT-qPCR) reaction mixture was developed. A simple and rapid RT-qPCR detection method for PRRSV was developed by optimizing nucleic acid amplification conditions. The results showed that the method was able to specifically detect PRRSV without cross-reactivity with the other 11 porcine susceptible viruses. The sensitivities of the assay were 3.12 × 10⁰ copies/μL and 10⁰ TCID₅₀/μL for M gene and virus, respectively, and the repeatability and reprodu

SUBMITTER: Zhao H 

PROVIDER: S-EPMC11768678 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature