Peptide Antigen Modifications Influence the On-Target and Off-Target Antibody Response for an Influenza Subunit Vaccine.

ABSTRACT:

Background/objectives

Peptide amphiphile micelles (PAMs) are an exciting nanotechnology currently being studied for a variety of biomedical applications, especially for drug delivery. Specifically, PAMs can enhance in vivo trafficking, cell-targeting, and cell interactions/internalization. However, modifying peptides, as is commonly performed to induce micellization, can influence their bioactivity. In our previous work, murine antibody responses to PAMs containing the influenza antigen M2_2-16 were slightly incongruous with prior PAM vaccine studies using other antigens. In this current work, the effect of native protein linkages and non-native micellizing moieties on M2 immunogenicity was studied.

Methods

PAMs were synthesized using an elongated M2 antigen (i.e.,

SUBMITTER: Schulte MC 

PROVIDER: S-EPMC11768957 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature