A Series of Novel Alleles of <i>Ehd2</i> Modulating Heading and Salt Tolerance in Rice.

ABSTRACT: Rice (Oryza sativa L.) is a staple crop for nearly half of the global population and one of China's most extensively cultivated cereals. Heading date, a critical agronomic trait, determines the regional and seasonal adaptability of rice varieties. In this study, a series of mutants (elh5 to elh12) exhibiting extremely late heading under both long-day (LD) and short-day (SD) conditions were identified from an ethyl methanesulfonate (EMS) mutant library. Using MutMap and map-based cloning, the causative gene was identified as a novel allele of Ehd2/OsID1/RID1/Ghd10. Functional validation through CRISPR/Cas9 knockout and complementation assays confirmed its role in regulating heading. The elh6 mutation was found to cause intron retention

SUBMITTER: Xu P 

PROVIDER: S-EPMC11769052 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature