Determination of Puberulic Acid in Monascus-Fermented Red Yeast Rice by LC-MS/MS Combined with Precolumn Derivatization.

ABSTRACT: Puberulic acid (PA) is a mycotoxin produced by a species of Penicillium. It has received widespread attention as a significant contributor to the reported fatalities associated with red yeast rice dietary supplements. However, the detection of PA, especially at low concentration levels, poses a considerable challenge, with no detection methods reported thus far. Here, we present a simple and sensitive derivatization-based LC-MS/MS method, requiring no purification processes, for determination of PA in the red yeast rice. The methylating derivatization with trimethylsilyldiazomethane (TMSCHN₂) was performed to enhance its analytical performance. To achieve optimal detection sensitivity, the amount of solvent and TMSCHN₂ for the derivatization reaction, along wit

SUBMITTER: Pan HQ 

PROVIDER: S-EPMC11769100 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature