The Effectiveness of Polyhydroxyalkanoate (PHA) Extraction Methods in Gram-Negative <i>Pseudomonas putida</i> U.

ABSTRACT: Bioplastics are emerging as a promising solution to reduce pollution caused by petroleum-based plastics. Among them, polyhydroxyalkanoates (PHAs) stand out as viable biotechnological alternatives, though their commercialization is limited by expensive downstream processes. Traditional PHA extraction methods often involve toxic solvents and high energy consumption, underscoring the need for more sustainable approaches. This study evaluated physical and chemical methods to extract PHAs from Pseudomonas putida U, a bacterium known to produce poly-3-hydroxyoctanoate P(3HO). Lyophilized cells underwent six extraction methods, including the use of the following: boiling, sonication, sodium hypochlorite (NaClO), sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and chloroform. Physica

SUBMITTER: Getino L 

PROVIDER: S-EPMC11769110 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature