Improving Sensitivity and Resolution of Dendrimer Identification in MALDI-TOF Mass Spectrometry Using Varied Matrix Combinations.

ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a well-known technique for polymer analysis, particularly for determining the molecular weight and structural details of dendrimers. In this study, we evaluated the performance of various matrices, such as 2',4',6'-trihydroxyacetophenone (THAP), α-cyano-4-hydroxycinnamic acid (HCCA), and sinapinic acid (SA), and their combinations, on the sensitivity and resolution of poly(amidoamine) (PAMAM) dendrimers of different generations (G3.0, G4.0, and G5.0). Our results demonstrated that the combination of HCCA-THAP significantly enhanced spectral resolution and peak intensity compared to individual matrices, particularly for higher-generation dendrimers. This improvement is attributed to the better ion

SUBMITTER: Sanhueza C 

PROVIDER: S-EPMC11769133 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature