Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity.

ABSTRACT:

Background

Available assays to measure pox virus neutralizing antibody titers are laborious and take up to 5 days. In addition, assays to measure T cell responses require the use of specific antigens, which may not be the same for all pox viruses. This study reports the development of robust assays for the measurement of mpox-specific neutralizing antibodies and IFN-γ-producing T-cell responses.

Methods

Fourteen samples from 7 volunteers who received Modified Vaccinia Ankara-Bavarian Nordic (MVA-BN) were used. The focused reduction neutralization test (FRNT) was performed using the mpox-specific A29 monoclonal antibody. Optimization and further development of FRNT were conducted using the plaque reduction neutralization test (PRNT) as the gold standard. The mpox-specific IFN

SUBMITTER: Yu Y 

PROVIDER: S-EPMC11769350 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature