T4 Phage Displaying Dual Antigen Clusters Against H3N2 Influenza Virus Infection.

ABSTRACT:

Background

The current H3N2 influenza subunit vaccine exhibits weak immunogenicity, which limits its effectiveness in preventing and controlling influenza virus infections.

Methods

In this study, we aimed to develop a T4 phage-based nanovaccine designed to enhance the immunogenicity of two antigens by displaying the HA1 and M2e antigens of the H3N2 influenza virus on each phage nanoparticle. Specifically, we fused the Soc protein with the HA1 antigen and the Hoc protein with the M2e antigen, assembling them onto a T4 phage that lacks Soc and Hoc proteins (Soc^-Hoc^-T4), thereby constructing a nanovaccine that concurrently presents both HA1 and M2e antigens.

Results

The analysis of the optical density of the target protein bands indicated that each

SUBMITTER: Liu S 

PROVIDER: S-EPMC11769387 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature