Importance of OsRac1 in Signalling of Pigm-1 Mediated Resistance to Rice Blast Disease.

ABSTRACT: In rice, leucine-rich repeat nucleotide-binding site (NLR) proteins are pivotal immune receptors in combating Magnaporthe oryzae-triggered rice blast. However, the precise molecular mechanism underlying how NLR proteins regulate downstream signalling remains elusive due to the lack of knowledge regarding their direct downstream targets. The NLR protein Pigm-1 was cloned from Shuangkang 77009 in our laboratory. This study shows that the nucleotide-binding site (NBS) domain of Pigm-1 facilitates its binding to and activation of OsRac1 while the coiled-coil (CC) domain enables its binding to and activation of RAI1, ultimately inducing cell death. At the same time, after knocking out OsRac1 in the background of Shuangkang 77009 containing Pigm-1, two knockout lines showed

SUBMITTER: Yang D 

PROVIDER: S-EPMC11769553 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature