Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding <i>opaque2</i>, <i>opaque16</i> and <i>waxy1</i> genes in maize.

ABSTRACT: To explore the molecular mechanism behind maize grain quality and use of different gene stacking to improve the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing o2o16wx, along with the double-recessive mutant lines containing o2o16, o2wx, and o16wx. The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (o2o16wx, o2wx, and o16wx) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis. In the lysine degradation pathway, 'QCL8010_1' (o2o16wx) revealed that the gene e

SUBMITTER: Wu P 

PROVIDER: S-EPMC11769590 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature