EstiMAge: development of a DNA methylation clock to estimate the methylation age of single cells.

ABSTRACT:

Motivation

Since their introduction about 10 years ago, methylation clocks have provided broad insights into the biological age of different species, tissues, and in the context of several diseases or aging. However, their application to single-cell methylation data remains a major challenge, because of the inherent sparsity of such data, as many CpG sites are not covered. A methylation clock applicable on single-cell level could help to further disentangle the processes that drive the ticking of epigenetic clocks.

Results

We have developed estiMAge ("estimation of Methylation Age"), a framework that exploits redundancy in methylation data to substitute missing CpGs of trained methylation clocks in single cells. Using Euclidean distance as a measure of similarity, we determi

SUBMITTER: Saßmannshausen Z 

PROVIDER: S-EPMC11769677 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature