Effects of acteoside from <i>Cistanche tubulosa</i> on the plasma metabolome of cancer-related fatigue mice inoculated with colon cancer cells.

ABSTRACT:

Objective

To elucidate the metabolic mechanisms by which acteoside (ACT) isolated from Cistanche tubulosa alleviates cancer-related fatigue (CRF) in a murine model of colon cancer with cachexia.

Methods

BALB/c mice inoculated with C26 colon cancer cells were treated with paclitaxel (PTX, 10 mg/kg) and ACT (100 mg/kg) alone or in combination for 21 days. Fatigue-associated behaviors, tumor inhibition rate, and skeletal muscle morphology assessed by hematoxylin-eosin (H&E) staining and electron microscopy were evaluated. Finally, liquid chromatography-mass spectrometry (LC/MS) was employed to investigate alterations in the plasma metabolic profile of tumor-bearing mice with CRF in response to ACT treatment, and the affinity between metabolite-associated proteins and ACT

SUBMITTER: Zhang S 

PROVIDER: S-EPMC11769790 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature