Improved plant biomass production under low nitrogen conditions through conditional accumulation of the second messenger, guanosine tetraphosphate, in chloroplasts and mitochondria.

ABSTRACT: To enhance plant biomass production under low nitrogen conditions, we employed a method to artificially and temporarily accumulate the bacterial second messenger, guanosine tetraphosphate (ppGpp), to modify plastidial or mitochondrial metabolism. Specifically, we fused a chloroplast or mitochondrial transit-peptide to the N-terminus of the bacterial ppGpp synthase YjbM, which was conditionally expressed by an estrogen-inducible promoter in Arabidopsis. The resulting recombinant Arabidopsis plants exhibited estrogen-dependent ppGpp accumulation in chloroplasts or mitochondria and showed reduced fresh weight compared to wild type (WT) plants when grown on agar-solidified plates containing a certain amount of estrogen. This finding aligns with the previous study indicating that

SUBMITTER: Goto M 

PROVIDER: S-EPMC11770007 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature