Specific immune response to <i>M. tuberculosis</i> and ability to <i>in vitro</i> control mycobacterial replication are not impaired in subjects with immune-mediated inflammatory disease and tuberculosis infection.

ABSTRACT:

Background

Subjects with immune-mediated inflammatory diseases (IMID), such as rheumatoid arthritis, with tuberculosis infection (TBI), have a high probability of progressing to tuberculosis disease (TB). We aim to characterize the impact of IMID on the immune response to M. tuberculosis (Mtb) in patients with TBI and TB disease.

Methods

We enrolled TBI and TB patients with and without IMID. Peripheral blood mononuclear cells (PBMCs) were stimulated with Mtb-derived epitopes (MTB300). By flow-cytometry, we identified the Mtb-specific CD4⁺ T cells as cytokine-producing T cells or as CD25⁺ CD134⁺ CD4⁺ T cells. Memory and activation status of Mtb-specific T cells were assessed by evaluating: CD153, HLA-DR, CD45RA, CD27. Mycobac

SUBMITTER: Farroni C 

PROVIDER: S-EPMC11770028 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature