Modulation of microtubule dynamics by monovalent ions.

ABSTRACT: The microtubule cytoskeleton is a dynamic network essential for many cellular processes, influenced by physicochemical factor, such as temperature, pH, dimer concentration, and ionic environment. In this study, we used in vitro reconstitution assays to examine the effects of four monovalent ions (Na⁺, K⁺, Cl^-, and Ac^-) on microtubule dynamics, uncovering distinct effects for each ion. Na⁺ was found to increase microtubule dynamicity by raising catastrophe frequency, polymerization and depolymerization speeds, and ultimately reducing microtubule lifetime by 80%. Conversely, Ac^- boosts microtubule nucleation and stabilizes microtubules by increasing rescue frequency and preventing breakages, resulting in longer microtubules wi

SUBMITTER: Fernandes S 

PROVIDER: S-EPMC11770338 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature