Computational discovery of co-expressed antigens as dual targeting candidates for cancer therapy through bulk, single-cell, and spatial transcriptomics.

ABSTRACT:

Motivation

Bispecific antibodies (bsAbs) that bind to two distinct surface antigens on cancer cells are emerging as an appealing therapeutic strategy in cancer immunotherapy. However, considering the vast number of surface proteins, experimental identification of potential antigen pairs that are selectively expressed in cancer cells and not in normal cells is both costly and time-consuming. Recent studies have utilized large bulk RNA-seq databases to propose bispecific targets for various cancers. However, co-expressed pairs derived from bulk RNA-seq do not necessarily indicate true co-expression of both markers in malignant cells. Single-cell RNA-seq (scRNA-seq) can circumvent this issue but the issues in low coverage of transcripts impede the large-scale characterization of co-ex

SUBMITTER: Chekalin E 

PROVIDER: S-EPMC11770384 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature