Deficiency in homologous recombination is associated with changes in cell cycling and morphology in Saccharomyces cerevisiae.

ABSTRACT: Exposure of eukaryotic cells to ionizing radiation or clastogenic chemicals leads to formation of DNA double-strand breaks (DSBs). These lesions are also generated internally by chemicals and enzymes, in the absence of exogenous agents, though the sources and consequences of such endogenously generated DSBs remain poorly understood. In the current study, we have investigated the impact of reduced recombinational repair of endogenous DSBs on stress responses, cell morphology and other physical properties of S. cerevisiae (budding yeast) cells. Use of phase contrast and DAPI-based fluorescence microscopy combined with FACS analysis confirmed that recombination-deficient rad52 cell cultures exhibit chronically high levels of G₂ phase cells. Cell cycle phase transit times during G

SUBMITTER: Holland CL 

PROVIDER: S-EPMC11770826 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature