Ribosome pausing in amylase producing Bacillus subtilis during long fermentation.

ABSTRACT:

Background

Ribosome pausing slows down translation and can affect protein synthesis. Improving translation efficiency can therefore be of commercial value. In this study, we investigated whether ribosome pausing occurs during production of the α-amylase AmyM by the industrial production organism Bacillus subtilis under repeated batch fermentation conditions.

Results

We began by assessing our ribosome profiling procedure using the antibiotic mupirocin that blocks translation at isoleucine codons. After achieving single codon resolution for ribosome pausing, we determined the genome wide ribosome pausing sites for B. subtilis at 16 h and 64 h growth under batch fermentation. For the highly expressed α-amylase gene amyM several strong ribosome pausing sites were detected, which

SUBMITTER: Han Y 

PROVIDER: S-EPMC11770953 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature