Getah virus triggers ROS-mediated autophagy in mouse Leydig cells.

ABSTRACT:

Introduction

Getah virus (GETV) is a zoonotic virus transmitted via a mosquito-vertebrate cycle. While previous studies have explored the epidemiology and pathogenicity of GETV in various species, its molecular mechanisms remain largely unexplored.

Methods

This study investigated the impact of GETV infection and associated molecular mechanisms on reactive oxygen species (ROS) and autophagy levels in mouse Leydig cells both in vivo and in vitro. The male mice and TM3 cells were treatment with N-acetylcysteine (NAC) to reduce cellular ROS levels. Rapamycin (Rapa) and 3-Methyladenine (3- MA) were used to change autophagy in both infected and uninfected TM3 cells.

Results and discussion

The findings revealed that GETV infection in mouse testes speciffcally t

SUBMITTER: Li F 

PROVIDER: S-EPMC11771000 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature