Metatranscriptomic time series insight into antibiotic resistance genes and mobile genetic elements in wastewater systems under antibiotic selective pressure.

ABSTRACT:

Background

Wastewater systems are usually considered antibiotic resistance hubs connecting human society and the natural environment. Antibiotic usage can increase the abundance of both ARGs (antibiotic resistance genes) and MGEs (mobile gene elements). Understanding the transcriptomic profiles of ARGs and MGEs remains a major research goal.

Methods

Batch experiments were conducted by adding ampicillin to fresh activated sludge to final concentrations of 100 and 20 mg/L. The ampicillin concentration was detected via UPLC‒MS/MS. Metatranscriptomic data from the samples in the reactors were detected in a time series (0 to 10 h). The transcription profiles of ARGs and MGEs were summarized by searching metatranscriptomic datasets against related databases.

Results

During

SUBMITTER: Li AD 

PROVIDER: S-EPMC11771064 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature