Characterization and effective expansion of CD4^-CD8^- TCRαβ⁺ T cells from individuals living with type 1 diabetes.

ABSTRACT: CD4^-CD8^- TCRαβ⁺ (double-negative [DN]) T cells represent a rare T cell population that promotes immunological tolerance through various cytotoxic mechanisms. In mice, autologous transfer of DN T cells has shown protective effects against autoimmune diabetes and graft-versus-host disease. Here, we characterized human DN T cells from people living with type 1 diabetes (PWT1D) and healthy controls. We found that while DN T cells and CD8⁺ T cells share many similarities, DN T cells are a unique T cell population, both at the transcriptomic and protein levels. We also show that by using various cytokine combinations, human DN T cells can be expanded in vitro up to 1,000-fold (mean >250-fold) and remain functional post-expansion. In addition, we

SUBMITTER: Fajardo-Despaigne JE 

PROVIDER: S-EPMC11772147 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature