Novel biomarkers of preterm brain injury from blood transcriptome in sheep model of intrauterine asphyxia.

ABSTRACT:

Background

Infants born preterm have a higher incidence of neurological deficits. A key step in finding effective treatments is to identify biomarkers that reliably predict outcome.

Methods

Following umbilical cord occlusion (UCO) in pregnant sheep, whole fetal blood RNA was sequenced pre- and post-UCO, brain injury outcome was determined by battery of neuropathology scoring and the transcriptome signature correlated to the degree of brain injury. Additionally, we developed a novel analytical procedure to deduce cell blood composition over time.

Results

Sixty-one genes were identified with significant altered expression after UCO. In pre-UCO blood, the level of three mRNAs (Trex2, Znf280b, novel miRNA) and in post-UCO, four mRNAs (Fam184a, Angptl2, novel lincRNA and a

SUBMITTER: Ek CJ 

PROVIDER: S-EPMC11772238 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature