Metabolic engineering of narrow-leafed lupin for the production of enantiomerically pure (-)-sparteine.

ABSTRACT: The protein crops known as lupins have been bred to accumulate low levels of antinutritional alkaloids, neglecting their potential as sources of valuable metabolites. Here, we engineered narrow-leafed lupin (NLL) to accumulate large amounts of a single alkaloid of industrial interest called (-)-sparteine. While (-)-sparteine is recognized as a key auxiliary molecule in chiral synthesis, its variable price and limited availability have prevented its large-scale use. We identified two enzymes that initiate the conversion of (-)-sparteine to a variety of alkaloids accumulating in NLL. The first one is a cytochrome P450 monooxygenase belonging to family 71 (CYP71D189), and the second one is a short-chain dehydrogenase/reductase (SDR1). We screened a non-GMO NLL mutant library and isolated a kn

SUBMITTER: Mancinotti D 

PROVIDER: S-EPMC11772310 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature