Increasing thermostability of the key photorespiratory enzyme glycerate 3-kinase by structure-based recombination.

ABSTRACT: As global temperatures rise, improving crop yields will require enhancing the thermotolerance of crops. One approach for improving thermotolerance is using bioengineering to increase the thermostability of enzymes catalysing essential biological processes. Photorespiration is an essential recycling process in plants that is integral to photosynthesis and crop growth. The enzymes of photorespiration are targets for enhancing plant thermotolerance as this pathway limits carbon fixation at elevated temperatures. We explored the effects of temperature on the activity of the photorespiratory enzyme glycerate kinase (GLYK) from various organisms and the homologue from the thermophilic alga Cyanidioschyzon merolae was more thermotolerant than those from mesophilic plants, including Arabidopsis th

SUBMITTER: Roze LV 

PROVIDER: S-EPMC11772331 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature