Non-specific effect of double-stranded RNAs on Egyptian broomrape (<i>Phelipanche aegyptiaca</i>) seed germination.

ABSTRACT: Obligate root parasitic plants of the Orobanchaceae family exhibit an intricate germination behavior. The host-dependent germination process of these parasites has prompted extensive research into effective control methods. While the effect of biomaterials such as amino acids and microRNA-encoded peptides have been explored, the effect of double-stranded RNAs (dsRNAs) has remained unexamined during the germination process. In this study, we asked whether an exogenously applied dsRNA can inhibit the germination of a root parasite, P. aegyptiaca. To this end, a dsRNA was designed to target the CYP707A1 (dsCYP7), a marker gene of the chemically-dependent germination of broomrape seeds. Application of a concentrated dsCYP7 significantly reduced seed germination. However, two non-

SUBMITTER: Zainali N 

PROVIDER: S-EPMC11772421 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature