A novel label-free method to determine equilibrium dissociation constants of antibodies binding to cell surface proteins.

ABSTRACT: Solution-based affinity assays are used for the selection and characterization of proteins that could be developed into therapeutic molecules. However, these assays have limitations for cell-surface proteins as in most cases their purification requires detergent solubilization and are unlikely to assume conformations in solution that resemble their native states in cell membranes. This report describes a novel electrochemiluminescence-based method, called MSD-CAT, for the affinity analysis of antibodies binding to cell-surface receptors. MSD-CAT was used to evaluate the binding of monoclonal antibodies, Fab fragments, and bispecific antibodies targeting the cell-surface receptor interleukin 3 receptor alpha (CD123) and the results were compared to data obtained using surface plasmon resona

SUBMITTER: Lacy ER 

PROVIDER: S-EPMC11772844 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature