The role of ER exit sites in maintaining P-body organization and integrity during Drosophila melanogaster oogenesis.

ABSTRACT: Processing bodies (P-bodies) are cytoplasmic membrane-less organelles which host multiple mRNA processing events. While the fundamental principles of P-body organization are beginning to be elucidated in vitro, a nuanced understanding of how their assembly is regulated in vivo remains elusive. Here, we investigate the potential link between ER exit sites and P-bodies in Drosophila melanogaster egg chambers. Employing a combination of live and super-resolution imaging, we find that P-bodies associated with ER exit sites are larger and less mobile than cytoplasmic P-bodies, indicating that they constitute a distinct class of P-bodies. Moreover, we demonstrate that altering the composition of ER exit sites has differential effects on core P-body proteins (Me31B, Cup, and Trailer Hitch), sugge

SUBMITTER: Milano SN 

PROVIDER: S-EPMC11772875 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature