Mechanisms of axoneme and centriole elimination in Naegleria gruberi.

ABSTRACT: The early branching eukaryote Naegleria gruberi can transform transiently from an amoeboid life form lacking centrioles and flagella to a flagellate life form where these elements are present, followed by reversion to the amoeboid state. The mechanisms imparting elimination of axonemes and centrioles during this reversion process are not known. Here, we uncover that flagella primarily fold onto the cell surface and fuse within milliseconds with the plasma membrane. Once internalized, axonemes are severed by Spastin into similarly-sized fragments that are then enclosed by membranes, before their contents are eliminated through the lysosomal pathway. Moreover, we discovered that centrioles undergo progressive K63 autophagy-linked poly-ubiquitination and K48 proteasome-promoting poly-ubiquiti

SUBMITTER: Woglar A 

PROVIDER: S-EPMC11772885 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature