Upregulation of interferon-γ response genes in monocytes and T cells identified by single-cell transcriptomics in patients with anti-citrullinated peptide antibody-positive early rheumatoid arthritis.

ABSTRACT:

Introduction

Our aim was to investigate the insufficiently understood differences in the immune system between anti-citrullinated peptide antibody (ACPA)-positive (ACPA⁺) and ACPA-negative (ACPA^-) early rheumatoid arthritis (eRA) patients.

Methods

We performed multiple cytokine assays using sera from drug-naïve ACPA⁺ and ACPA^- eRA patients. Additionally, we conducted single-cell RNA sequencing of CD45⁺ cells from peripheral blood samples to analyze and compare the distribution and functional characteristics of the cell subsets based on the ACPA status.

Results

Serum concentrations of interferon-γ (IFN-γ) and interleukin (IL)-12 were higher in ACPA⁺ eRA than in ACPA^- eRA. Single-cell transcrip

SUBMITTER: Hong BK 

PROVIDER: S-EPMC11772891 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature