Electromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis.

ABSTRACT: Multiphasic buffer systems have been of greatest interest in electrophoresis and liquid-liquid electrotransfer; this study extends that foundation by exploring the interplay of the geometric and viscous properties of an interleaving oil layer on the electrotransfer of a charged analyte from an aqueous solution into a hydrogel. We utilized finite element analysis to examine two complementary configurations: one being electrotransfer of a charged analyte (protein) in an aqueous phase into a surrounding hydrogel layer and another being electrotransfer of the protein from that originating aqueous phase-through an interleaving oil layer of predetermined viscosity and thickness-and into a surrounding hydrogel layer. Results indicate that the presence of an oil layer leads to increased skew of th

SUBMITTER: Islam MN 

PROVIDER: S-EPMC11773303 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature