Investigation into the effect of phenylalanine gating on anaerobic haem breakdown using the energy landscape approach.

ABSTRACT: We have recently demonstrated a novel anaerobic NADH-dependent haem breakdown reaction, which is carried out by a range of haemoproteins. The Yersinia enterocolitica protein, HemS, is the focus of further research presented in the current paper. Using conventional experimental methods, bioinformatics, and energy landscape theory (ELT), we provide new insight into the mechanism of the novel breakdown process. Of particular interest is the behavior of a double phenylalanine gate, which opens and closes according to the relative situations of haem and NADH within the protein pocket. This behavior suggests that the double phe-gate fulfills a regulatory role within the pocket, controlling the access of NADH to haem. Additionally, stopped-flow spectroscopy results provide kinetic comparisons bet

SUBMITTER: Keith AD 

PROVIDER: S-EPMC11773379 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature