Development of a qPCR assay to identify and differentiate insect-associated strains of the <i>Serratia marcescens</i> complex.

ABSTRACT: The Serratia marcescens complex contains important opportunistic pathogens of humans and vertebrate animals, as well as insects and other invertebrates. To date, the methods used for the identification of species within the genus Serratia, including PCR assays, have poor discriminatory power and may require further molecular typing or genomic sequence analysis to determine clinical relevance. We developed a duplex TaqMan probe-based quantitative real-time PCR (qPCR) assay targeting the chiP gene, which is involved in chitin degradation and transport, and the ureD gene, which is involved in urease production. This allowed us to simultaneously identify all members of the S. marcescens complex (chiP positive) and differentiate those most likely to act

SUBMITTER: Doidge NP 

PROVIDER: S-EPMC11773504 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature