NADPH-Independent Fluorescent Probe for Live-Cell Imaging of Heme Oxygenase-1.

ABSTRACT: Heme oxygenase-1 (HO-1) catalyzes heme degradation on the consumption of NADPH and molecular oxygen. As an inducible enzyme, HO-1 is highly induced in various disease states, including cancer. Currently, two fluorescent probes for HO-1 have been designed based on the catalytic activity of HO-1, in which the probes serve as a substrate, so NADPH is required to enable the detection. Probes functioning in a NADPH-dependent way may influence other NADPH-consuming pathways, as all these pathways share a common NADPH pool. Here, we report the peptide-based fluorescent probe NBD-P₅ as a simple alternative approach for HO-1 sensing. The designed probe NBD-P₅ functions independently of the catalytic activity of HO-1, therefore enabling fast and sensitive detection of HO-1 with

SUBMITTER: Li L 

PROVIDER: S-EPMC11773557 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature