Detection of human noroviruses in sewage by next generation sequencing in Shandong Province, 2019-2021.

ABSTRACT:

Background

Human noroviruses are the major cause of acute gastroenteritis and exhibit considerable genetic diversity. Next generation sequencing (NGS) analysis based on environmental surveillance has been proved to be an effective method in norovirus surveillance.

Methods

Between January 2019 and December 2021, 36 sewage samples were collected and analyzed using real-time quantitative PCR to detect noroviruses. Partial VP1 region was amplified and subjected to NGS analysis to assess the abundance and genetic characterization of various norovirus genotypes across different samples.

Results

A total of 23 norovirus genotypes were identified, including 9 genotypes of GI, 13 genotypes of GII and 1 genotype of GIX. The most frequently detected genotypes were GI.5 (86.11%),

SUBMITTER: Wang S 

PROVIDER: S-EPMC11773704 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature