Single-cell Raman and fluorescence microscopy reveal the association of lipid bodies with phagosomes in leukocytes.

ABSTRACT: Cellular imaging techniques based on vibrational spectroscopy have become powerful tools in cell biology because the molecular composition of subcellular compartments can be visualized without the need for labeling. Using high-resolution, nonresonant confocal Raman microscopy on individual cells, we demonstrate here that lipid bodies (LBs) rich in arachidonate as revealed by their Raman spectra associate with latex bead-containing phagosomes in neutrophilic granulocytes. This finding was corroborated in macrophages and in PLB-985 cells, which can be induced to differentiate into neutrophil-like cells, by selective staining of LBs and visualization by confocal fluorescence microscopy. We further show that the accumulation of LBs near phagosomes is mediated at least in part by the flavohemop

SUBMITTER: van Manen HJ 

PROVIDER: S-EPMC1177376 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature