LncRNA-ANRIL regulates CDKN2A to promote malignant proliferation of Kasumi-1 cells.

ABSTRACT:

Objective

This study aimed to investigate the regulatory effects of long non-coding RNA-ANRIL on CDKN2A in the cell cycle of Kasumi-1 cells and elucidate the underlying molecular mechanisms.

Methods

ANRIL and CDKN2A expression levels were quantified using RT-qPCR in peripheral blood samples from acute myeloid leukemia (AML) patients. CDKN2A knockdown efficiency was validated via RT-qPCR, and cell cycle distribution was analyzed using flow cytometry. Cell proliferation assays were conducted with CCK-8 following palbociclib treatment and CDKN2A downregulation. RNA immunoprecipitation (RIP) identified potential ANRIL-associated targets, while western blotting assessed the expression levels of GSK3β/β-catenin/cyclin D1 signaling components and related proteins.

Results

AN

SUBMITTER: Xu J 

PROVIDER: S-EPMC11773856 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature