The quest for environmental analytical microbiology: absolute quantitative microbiome using cellular internal standards.

ABSTRACT:

Background

High-throughput sequencing has revolutionized environmental microbiome research, providing both quantitative and qualitative insights into nucleic acid targets in the environment. The resulting microbial composition (community structure) data are essential for environmental analytical microbiology, enabling characterization of community dynamics and assessing microbial pollutants for the development of intervention strategies. However, the relative abundances derived from sequencing impede comparisons across samples and studies.

Results

This review systematically summarizes various absolute quantification (AQ) methods and their applications to obtain the absolute abundance of microbial cells and genetic elements. By critically comparing the strengths and limitatio

SUBMITTER: Wang C 

PROVIDER: S-EPMC11773863 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature