Cell signaling communication within papillary craniopharyngioma revealed by an integrated analysis of single-cell RNA sequencing and bulk RNA sequencing.

ABSTRACT:

Objective

This study aims to elucidate the primary signaling communication among papillary craniopharyngioma (PCP) tumor cells.

Methods

Five samples of PCP were utilized for single-cell RNA sequencing. The most relevant ligand and receptor interactions among different cells were calculated using the CellChat package in R software. Bulk RNA sequencing of 11 tumor samples and five normal controls was used to investigate the pair interactions detected by single-cell RNA sequencing.

Results

Fibroblasts were not found in ACP, whereas they were detected in PCP. InferCNV revealed high CNV scores for the clusters of epithelial cells and fibroblasts using immune cells as a reference. Epithelial Mesenchymal Transition, Interferon Gamma Response, p53 Pathway, and Estrogen Respon

SUBMITTER: Zhu X 

PROVIDER: S-EPMC11773883 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature