Preliminary clinical performance of a Cas13a-based lateral flow assay for detecting <i>Neisseria gonorrhoeae</i> in urine specimens.

ABSTRACT: Nucleic acid amplification testing (NAAT) for N. gonorrhoeae is unavailable in resource-limited settings. We previously developed a CRISPR-based lateral flow assay for detecting N. gonorrhoeae. We aimed to pair that assay with point-of-care DNA extraction, assess performance in clinical urine specimens, and optimize assay kinetics. We collected urine specimens among men presenting with urethritis enrolling in a clinical trial at the Massachusetts General Hospital Sexual Health Clinic. We assessed the quantified DNA yield of detergent-based extractions with and without heat. We selected one detergent for extracting all specimens, paired with isothermal recombinase polymerase amplification for 90 minutes and lateral flow Cas13a detection, interpreted via pixel intensity analysi

SUBMITTER: Allan-Blitz L-T 

PROVIDER: S-EPMC11774021 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature