Identification of novel fructo-oligosaccharide bacterial consumers by pulse metatranscriptomics in a human stool sample.

ABSTRACT: Dietary fibers influence the composition of the human gut microbiota and directly contribute to its downstream effects on host health. As more research supports the use of glycans as prebiotics for therapeutic applications, the need to identify the gut bacteria that metabolize glycans of interest increases. Fructo-oligosaccharide (FOS) is a common diet-derived glycan that is fermented by the gut microbiota and has been used as a prebiotic. Despite being well studied, we do not yet have a complete picture of all FOS-consuming gut bacterial taxa. To identify new bacterial consumers, we used a short exposure of microbial communities in a stool sample to FOS or galactomannan as the sole carbon source to induce glycan metabolism genes. We then performed metatranscriptomics, paired with whole me

SUBMITTER: Prattico C 

PROVIDER: S-EPMC11774028 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature