Melatonin improves the in vitro growth of bovine oocytes collected from early antral follicles by maintaining oocyte-cumulus cell communication.

ABSTRACT:

Purpose

In vitro, oocyte development is susceptible to oxidative stress, which leads to endoplasmic reticulum (ER) stress. This study investigated whether the antioxidant melatonin attenuates ER stress and maintains oocyte-cumulus cell communication during the in vitro growth (IVG) of bovine oocytes.

Methods

Oocyte-granulosa cell complexes (OGCs) were harvested from slaughterhouse-derived ovaries and grown in vitro for 5 d at 38.5°C in 5% CO₂ humidified air. Melatonin (10^-7, 10^-9, or 10^-11 M) was added to the culture medium.

Results

Oocyte diameter increased on day 5 from its initial value in all groups. The antrum formation rate was significantly higher in the 10^-9 M melatonin-treated group than in the control. T

SUBMITTER: Islam MN 

PROVIDER: S-EPMC11774242 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature