Characterization of a Monoclonal Antibody by Native and Denaturing Top-Down Mass Spectrometry.

ABSTRACT: Established in recent years as an important approach to unraveling the heterogeneity of intact monoclonal antibodies, native mass spectrometry has been rarely utilized for sequencing these complex biomolecules via tandem mass spectrometry. Typically, top-down mass spectrometry has been performed starting from highly charged precursor ions obtained via electrospray ionization under denaturing conditions (i.e., in the presence of organic solvents and acidic pH). Here we systematically benchmark four distinct ion dissociation methods─namely, higher-energy collisional dissociation, electron transfer dissociation, electron transfer dissociation/higher-energy collisional dissociation, and 213 nm ultraviolet photodissociation─in their capability to characterize a therapeutic monoclonal antibody,

SUBMITTER: Oates RN 

PROVIDER: S-EPMC11774622 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature