Identification of Critical Phosphorylation Sites Enhancing Kinase Activity With a Bimodal Fusion Framework.

ABSTRACT: Phosphorylation is an indispensable regulatory mechanism in cells, with specific sites on kinases that can significantly enhance their activity. Although several such critical phosphorylation sites (phos-sites) have been experimentally identified, many more remain to be explored. To date, no computational method exists to systematically identify these critical phos-sites on kinases. In this study, we introduce PhoSiteformer, a transformer-inspired foundational model designed to generate embeddings of phos-sites using phosphorylation mass spectrometry data. Recognizing the complementary insights offered by protein sequence data and phosphorylation mass spectrometry data, we developed a classification model, CSPred, which employs a bimodal fusion strategy. CSPred combines embeddings from Pho

SUBMITTER: Zhang M 

PROVIDER: S-EPMC11774822 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature