Global insights into the genome dynamics of <i>Clostridioides difficile</i> associated with antimicrobial resistance, virulence, and genomic adaptations among clonal lineages.

ABSTRACT:

Background

Clostridioides difficile is a significant cause of healthcare-associated infections, with rising antimicrobial resistance complicating treatment. This study offers a genomic analysis of C. difficile, focusing on sequence types (STs), global distribution, antibiotic resistance genes, and virulence factors in its chromosomal and plasmid DNA.

Methods

A total of 19,711 C. difficile genomes were retrieved from GenBank. Prokka was used for genome annotation, and multi-locus sequence typing (MLST) identified STs. Pan-genome analysis with Roary identified core and accessory genes. Antibiotic resistance genes, virulence factors, and toxins were detected using the CARD and VFDB databases, and the ABRicate software. Statistical analyses and visualization

SUBMITTER: Sholeh M 

PROVIDER: S-EPMC11774869 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature