Development of a colloidal gold immunochromatographic assay utilizing dual-antibody sandwich method for detecting <i>Orientia tsutsugamushi</i>.

ABSTRACT: A colloidal gold immunochromatographic assay (ICA) based on a dual-antibody sandwich method was developed for the rapid and convenient detection of Orientia tsutsugamushi (O. tsutsugamushi) antigens in the early stages of infection. Monoclonal antibodies designed as 5B3 targeting the conserved region of 56 kDa outer membrane protein in various strains of O. tsutsugamushi were generated through cell fusion and screening techniques and combined with previously prepared polyclonal antibodies as detection antibodies to establish the ICA. Colloidal gold and polyclonal antibody-colloidal gold complexes were synthesized under optimized conditions. The nitrocellulose membrane was treated with 5B3 monoclonal antibody and goat anti-mouse antibody as the test and control lines, r

SUBMITTER: Lu Q 

PROVIDER: S-EPMC11774921 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature