Comparative characterization of organ-specific phase I and II biotransformation enzyme kinetics in salmonid S9 sub-cellular fractions and cell lines.

ABSTRACT: Advancing in vitro systems to address the effects of chemical pollution requires a thorough characterization of their functionalities, such as their repertoire of biotransformation enzymes. Currently, knowledge regarding the presence, activity magnitudes, and inducibility of different biotransformation pathways in vitro is scarce, particularly across organs. We report organ-specific kinetics for phase I and II biotransformation enzymes, under basal and induced conditions, in two in vitro systems using salmonid fish: S9 sub-cellular fractions from brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) were compared with rainbow trout cell lines. Cyp1a and glutathione S-transferase (Gst) activities were the highest in liver S9 fractions and RTL-W1 liver cells, yet systems derived

SUBMITTER: Martin BPM 

PROVIDER: S-EPMC11775053 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature