Understanding the vaginal microbiome among women with different genotypes of human papillomavirus infection in remote Andaman islands.

ABSTRACT:

Background

Human papillomavirus (HPV) is a viral infection, and its acquisition and persistence are significantly influenced by the vaginal microbiota. Understanding and comparing the vaginal microbiome of HPV infected women in Andaman and Nicobar Islands is crucial.

Methods

The study involved collecting vaginal swabs and extracting DNA using the QIAamp DNA Minikit. The DNA was then subjected to PCR amplification to confirm HPV infection. illumina NovaSeq 6000 platform was utilized to perform sequencing utilizing 2 x 250 paired end chemistry. Taxonomic analysis was performed and Bacterial abundance plots were generated and samples were grouped based on demographic parameters, pap test diagnosis, and genotypes. To assess diversity, samples were rarefied to 49,000 sequence rea

SUBMITTER: Parvez R 

PROVIDER: S-EPMC11775162 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature