Single-cell transcriptomics of bronchoalveolar lavage during PRRSV infection with different virulence.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses in the global swine industry due to its high genetic diversity and different virulence levels, which complicate disease management and vaccine development. This study evaluated longitudinal changes in the immune cell composition of bronchoalveolar lavage fluid and the clinical outcomes across PRRSV strains with varying virulence, using techniques including single-cell transcriptomics. In highly virulent infection, faster viral replication results in an earlier peak lung-damage time point, marked by significant interstitial pneumonia, a significant decrease in macrophages, and an influx of lymphocytes. Viral tracking reveals less than 5% of macrophages are directly infected, and further analysis i

SUBMITTER: Lim B 

PROVIDER: S-EPMC11775223 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature