Sub-cellular population imaging tools reveal stable apical dendrites in hippocampal area CA3.

ABSTRACT: Apical and basal dendrites of pyramidal neurons receive anatomically and functionally distinct inputs, implying compartment-level functional diversity during behavior. To test this, we imaged in vivo calcium signals from soma, apical dendrites, and basal dendrites in mouse hippocampal CA3 pyramidal neurons during head-fixed navigation. To capture compartment-specific population dynamics, we developed computational tools to automatically segment dendrites and extract accurate fluorescence traces from densely labeled neurons. We validated the method on sparsely labeled preparations and synthetic data, predicting an optimal labeling density for high experimental throughput and analytical accuracy. Our method detected rapid, local dendritic activity. Dendrites showed robust spatial tuning, sim

SUBMITTER: Moore JJ 

PROVIDER: S-EPMC11775317 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature