Design and Validation of an Open-Close Device for Integrated Environmental DNA Sampling Detects A Depth Gradient in Indian Ocean Deep-Sea Fish Assemblages.

ABSTRACT: Advances in methods for collecting environmental DNA (eDNA) are revolutionizing biomonitoring capabilities. The goal of this study was to leverage existing survey technology to design and test an eDNA sampler that captures an integrated eDNA sample over the length of a deep-water transect. We manufactured a 300 × 100 × 100 mm mountable, open-ended box made of high-density polyethylene that could be attached to the frame of a preexisting deep tow camera system. The box (OCD; open-close device) was equipped with an actuator that attached to hinged doors at both ends, enabling it to be opened and closed remotely at depths up to 6000 m through preexisting communications, thereby exposing the internal chamber to the surrounding water upon activation. A sterile active carbon sponge was inserted

SUBMITTER: Bessey C 

PROVIDER: S-EPMC11775381 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature